Protein-trap version 2.1: screening for expressed proteins in mammalian cells based on their localizations.

Protein-trap version 2.1: screening for expressed proteins in mammalian cells based on their localizations.

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Abstract Background "Protein-trap" is a method that allows epitope-tagging of endogenous proteins.This method allows lovesense 3 for the identification of endogenously expressed proteins that exhibit specific localization of interest.This method has been recently reported for its application in the study of Drosophila development by using a relatively large epitope, green-fluorescent-protein (GFP).Result Herein, we report a new "protein-trap" vector for mammalian cells.This new method utilizes a much smaller epitope-tag and also southwestern aztec rug allows for drug-selection prior to the epitope-tagging.

Pre-selection by drug resulted in the highly efficient protein-trapping frequency.Conclusion The "protein-trap" method based on this new vector is expected to serve as a complimentary approach to the previously reported GFP-based method.